Direct Antiglobulin Test  (DAT or DAT CORD (cord blood))
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| Synonyms:
| Direct Coombs |
| Specimen: | Blood; (1) Purple (EDTA) top vacutainer tube. |
| Volume: | Full primary tube |
| Minimum Volume: | Full primary tube |
| Collection: | Perform test within 24 hours of collection.
|
| Rejection Criteria: | Incorrect specimen type; age of specimen; improper labeling. |
| Reference Range: | Negative |
| Methodology: | Manual Test Tube - Antiglobulin Test |
| Test Use: | Detects in vivo coating of IgG and/or complement on red blood cells |
| Additional Information: | Positive test associated with hemolytic transfusion reaction, auto immune disease, drug therapy or hemolytic disease of the newborn. |
| Testing Includes: | Tests red cells with polyspecific anti human globulin serum. Positive cells are further tested with monospecific anti-IgG. |
| Testing Facility: | AnMed Laboratory |